Human VAP-B Alexa Fluor® 488-conjugated Antibody

Discontinued Product

IC58551G has been discontinued.
Detection  of VAP‑B in T98G Human Cell Line by Flow Cytometry.
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Human VAP-B Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VAP-B in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human VAP-A is observed.
Source
Monoclonal Mouse IgG1 Clone # 736904
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VAP-B
Ala2-Pro132
Accession # O95292
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of VAP‑B in T98G Human Cell Line by Flow Cytometry. View Larger

Detection of VAP‑B in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Mouse Anti-Human VAP‑B Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC58551G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VAP-B

Vesicle-associated Membrane Protein (VAMP)-associated Protein B (VAP-B), also known as VAMP-B, is an ~30 Da ubiquitously expressed type IV transmembrane (TM) protein that belongs to the VAP family (1, 2). It is found in endoplasmic reticulum (ER), Golgi and other membranes as a homodimer or a heterodimer with VAP-A, probably associating through a GxxxG motif in the transmembrane regions (1, 2). Human VAP-B cDNA encodes 243 amino acids (aa) that include a 222 aa cytoplasmic domain and a 21 aa C-terminal membrane anchor. The cytoplasmic domain contains a mobile sperm protein (MSP) domain (aa 7‑124) plus a coiled-coil region (aa 159‑196) that initiates dimerization. Over aa 2-132, human VAP-B shares 97% aa sequence identity with mouse VAP-B and 81% aa sequence identity with VAP-A. VAP-A and VAP-B MSP domains recruit FFAT (two phenylalanines in an acidic tract)-motif-containing proteins to the cytosolic surface of ER membranes (2‑4). FFAT proteins mediate many of the effects of VAPs on regulation of membrane transport, phospholipid biosynthesis, microtubule organization, and the unfolded protein response (2, 3). VAPs also interact with some SNARE and viral proteins (2). A human polymorphism of VAP-B, P56S, is found in three familial motor neuron diseases, notably the amylotrophic lateral sclerosis variant ALS8 (2). It produces a non-functional protein that can dimerize with, and inhibit the function of, normal VAP-B, leading to formation of intracellular aggregates and increased ER-stress-induced death of motor neurons (5‑8). It can also promote cleavage and secretion of soluble VAP-B, which can then function as a ligand for EPH receptors (9). A naturally occurring 99 aa isoform of VAP-B that shows a 29 aa substitution for aa 71-243 is termed VAP-C (1, 10). It also appears to be a negative regulator of VAP-A and VAP-B (10). While VAP-B is used by hepatitis C virus (HCV) for its propagation, VAP-C inhibits HCV propagation (10).

References
  1. Nishimura, Y. et al. (1999) Biochem. Biophys. Res. Commun. 254:21.
  2. Lev, S. et al. (2008) Trends Cell Biol. 18:282.
  3. Peretti, D. et al. (2008) Mol. Biol. Cell 19:3871.
  4. Kaiser, S.E. et al. (2005) Structure 13:1035.
  5. Prosser, D.C. et al. (2008) J. Cell Sci. 121:3052.
  6. Gkogkas, C. et al. (2008) Hum. Mol. Genet. 17:1517.
  7. Suzuki, H. et al. (2009) J. Neurochem. 108:973.
  8. Kim, S. et al. (2010) J. Biol. Chem. 285:13839.
  9. Tsuda, H. et al. (2008) Cell 133:963.
  10. Kukihara, H. et al. (2009) J. Virol. 83:7959.
 
Long Name
VAMP [Vesicle-associated Membrane Protein]-associated Protein B and C
Entrez Gene IDs
9217 (Human); 56491 (Mouse); 60431 (Rat)
Alternate Names
ALS8; ALS8VAMP-B/VAMP-C; VAMP (vesicle-associated membrane protein)-associated protein B and C; VAMP-associated 33 kDa protein; VAMP-associated protein B/C; VAMP-B; VAMP-C; VAPB; VAP-B; VAP-B/VAP-C; VAPC; VAP-C; vesicle-associated membrane protein-associated protein B/C

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