Human Vav-1 Antibody Summary
Accession # P15498
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Vav‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Vav-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.
Citation for Human Vav-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Lipid rafts and caveolin-1 coordinate interleukin-1beta (IL-1beta)-dependent activation of NFkappaB by controlling endocytosis of Nox2 and IL-1beta receptor 1 from the plasma membrane.
Authors: Oakley FD, Smith RL, Engelhardt JF
J. Biol. Chem., 2009;284(48):33255-64.
Sample Types: Lipid Rafts
Applications: Western Blot
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