Human Vav-1 Antibody

R&D Systems | Catalog # AF3786

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Vav-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Vav-1
Trp25-Leu228
Accession # P15498

Specificity

Detects human Vav-1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Vav-1 Antibody

Detection of Human Vav‑1 antibody by Western Blot.

Detection of Human Vav‑1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Vav-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human Vav-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vav-1

The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.

Alternate Names

vav-T, Vav1

Entrez Gene IDs

7409 (Human); 22324 (Mouse); 25156 (Rat)

Gene Symbol

VAV1

UniProt

P15498

Additional Vav-1 Products

Product Documents for Human Vav-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Vav-1 Antibody

For research use only

Citations for Human Vav-1 Antibody

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