Human VCAM-1/CD106 Fluorescein-conjugated Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Source
    Monoclonal Mouse IgG2a Clone # BBIG-V3 (IE10)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    Please refer to the PDF datasheet for specifications.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Flow Cytometry

Anti-human VCAM-1/CD106 staining of HUT-78 cells using either isotype control (Catalog # IC003F; open histogram) or with anti-VCAM 1-Fluorescein (Catalog # BBA22; filled histogram).

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: VCAM-1/CD106

Human Vascular Cell Adhesion Molecule-1 (VCAM-1/CD106) is a 100 - 110 kDa, 715 amino acid, type I transmembrane glycoprotein (1 - 3). A number of variants of human VCAM-1 occur as a result of alternate gene splicing (1). Moreover, a soluble form of VCAM-1 has been identified in culture supernatants (4), blood (5 - 7) and cerebrospinal fluid (7, 8). Various proteases, including MMPs, neutrophil elastase, and cathepsin B have been implicated in the shedding of transmembrane VCAM-1 (9, 10). VCAM-1 is expressed constitutively on non-vascular cells including: dendritic cells in lymphoid tissues and skin (11), macrophages (11), fibroblasts (12), melanoma cells (11), smooth muscle cells (13), adult satellite muscle cells (14), bone marrow stromal cells (15), chondrocytes (16), mesothelium (17), renal tubular epithelium and mesangium (18), embryonic myoblasts and myotubules (14), neurons (19) and choroid plexus epithelium (20). VCAM-1 expression on vascular endothelium can be induced by a number of inflammatory stimuli (11).

Functionally, VCAM-1 mediates cell adhesion and signal transduction by binding to its ligands/counter-receptors. Ligands for VCAM-1 are the integrins alpha 41 (CD49d/CD29 or VLA4) and alpha 47 (11, 21 - 24), alpha D2 (25, 26), and alpha 91 (27). These VCAM-1 ligands are expressed on a variety of lymphoid cells. Accordingly, VCAM-1/VCAM-1 ligand interactions are key events in the rate and timing of leukocyte extravasation (11). Similarly, VCAM-1 mediates the adhesion of melanoma cells to endothelial cells and may play a role in metastasis (11). VCAM-1 expression on bone marrow stromal cells regulates T and B cell development as well as hematopoietic progenitor cell homing and trafficking (28 - 30). Other proposed roles for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism (15), and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia (31). Soluble VCAM-1 has been shown to mediate angiogenesis and is chemotactic for T lymphocytes and monocytes (11, 32).

  • Long Name:
    Vascular Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    7412 (Human); 22329 (Mouse)
  • Alternate Names:
    CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease
    Authors: SH Chan, CH Hung, JY Shih, PM Chu, YH Cheng, HC Lin, KL Tsai
    Redox Biol, 2017;13(0):301-309.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
    Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
    Stem Cells Dev, 2014;23(5):541-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Sinomenine suppresses TNF-alpha-induced VCAM-1 expression in human umbilical vein endothelial cells.
    Authors: Huang J, Lin Z, Luo M, Lu C, Kim MH, Yu B, Gu J
    J Ethnopharmacol, 2007;114(2):180-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
    Authors: Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R
    Atherosclerosis, 2007;190(1):90-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
    Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA
    Blood, 2007;109(1):122-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

FAQs

  1. What is the concentration of Human VCAM-1/CD106 Fluorescein-conjugated Antibody (Catalog # BBA22)?
    • The concentration of a fluorochrome-conjugated antibody may vary from lot to lot, and is listed on the left side of the Product Datasheet in the Reagents Provided section. Concentration is also listed on the lot-specific Certificate of Analysis (COA), which may be located by following the COA link on the product specific page. If you are unable to locate the concentration information, please contact Technical Service at techsupport@bio-techne.com or 1-800-343-7475.

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Fluorescein-conjugated Antibody

Ctrl IC003F 6  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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