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Human VCAM-1/CD106 Quantikine ELISA Kit

R&D Systems | Catalog # DVC00

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Key Product Details

Assay Length

2 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.26 ng/mL

Assay Range

6.25-200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human VCAM-1/CD106 Quantikine ELISA Kit

The Quantikine Human sVCAM-1 Immunoassay is a 2 hour solid-phase ELISA designed to measure human sVCAM-1 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sVCAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sVCAM-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human sVCAM-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 583 1423 2421 670 1571 2726
Standard Deviation 13.6 49.5 86.4 52.1 120.9 151.2
CV% 2.3 3.5 3.6 7.8 7.7 5.5

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of sVCAM-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human VCAM-1/CD106 ELISA Linearity

Scientific Data Images for Human VCAM-1/CD106 Quantikine ELISA Kit

Human VCAM-1/CD106 ELISA Standard Curve

Human VCAM-1/CD106 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VCAM-1/CD106

VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for Human VCAM-1/CD106 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VCAM-1/CD106 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human VCAM-1/CD106 Quantikine ELISA Kit

Customer Reviews for Human VCAM-1/CD106 Quantikine ELISA Kit (3)

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  • Human VCAM-1/CD106 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 01/27/2021
    Standard curve and results were in duplicate. Assay protocol was clear and concise.
    Human VCAM-1/CD106 Quantikine ELISA Kit DVC00
  • Human VCAM-1/CD106 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 01/27/2021
    Human VCAM-1/CD106 Quantikine ELISA Kit DVC00
  • Human sVCAM-1/CD106 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HPMEC
    Verified Customer | Posted 09/07/2017

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Protocols

View specific protocols for Human VCAM-1/CD106 Quantikine ELISA Kit (DVC00):

Refer to the product for complete assay procedure.
 
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
100 µL Assay Conjugate
100 µL Standard, Control, or Sample
100 µL Substrate Solution
50 µL Stop Solution
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
  3.   Add 100 µL of Assay Diluent to each well.
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
  6.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
  7.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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