Human VE-Cadherin Quantikine ELISA Kit

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Human VE-Cadherin ELISA Standard Curve
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Product Details
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Citations (4)
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Human VE-Cadherin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.236 ng/mL
Assay Range
1.6 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human VE-Cadherin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human VE-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VE-Cadherin in cell culture supernates, serum, and plasma. It contains NS0-derived recombinant human VE-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VE-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VE-Cadherin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 13.4 34.6 65.4 12.8 31.8 64.5
Standard Deviation 0.68 1.63 2.05 0.92 1.64 2.86
CV% 5.1 4.7 3.1 7.2 5.1 4.4

Recovery

The recovery of VE-Cadherin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 94-108

Linearity

To assess the linearity of the assay, samples containing high concentrations of VE-Cadherin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Data Example

Human VE-Cadherin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VE-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2+-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Long Name:
Vascular Endothelium Cadherin
Entrez Gene IDs:
1003 (Human); 12562 (Mouse)
Alternate Names:
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human VE-Cadherin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Ionizing radiation increases the endothelial permeability and the transendothelial migration of tumor cells through ADAM10-activation and subsequent degradation of VE-cadherin
    Authors: PN Kouam, GA Rezniczek, IA Adamietz, H Bühler
    BMC Cancer, 2019;19(1):958.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Recombinant erythropoietin acutely decreases renal perfusion and decouples the renin-angiotensin-aldosterone system
    Authors: NJ Aachmann-A, SJ Christense, K Lisbjerg, P Oturai, PI Johansson, NH Holstein-R, NV Olsen
    Physiol Rep, 2018;6(5):.
    Species: Human
    Sample Types: Serum
  3. Association of Endothelial Glycocalyx and Tight and Adherens Junctions With Severity of Plasma Leakage in Dengue Infection
    Authors: S Suwarto, RT Sasmono, R Sinto, E Ibrahim, M Suryamin
    J. Infect. Dis., 2017;215(6):992-999.
    Species: Human
    Sample Types: Serum
  4. How biomarkers reflect the prognosis and treatment of necrotising soft tissue infections and the effects of hyperbaric oxygen therapy: the protocol of the prospective cohort PROTREAT study conducted at a tertiary hospital in Copenhagen, Denmark
    Authors: P Polzik, PI Johansson, O Hyldegaard
    BMJ Open, 2017;7(10):e017805.
    Species: Human
    Sample Types: Plasma

FAQs

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Reviews for Human VE-Cadherin Quantikine ELISA Kit

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Human VE-Cadherin Quantikine ELISA Kit
By Anonymous on 11/22/2021
Sample Tested: human blood

Human VE-Cadherin Quantikine ELISA Kit
By Anonymous on 09/23/2021
Sample Tested: Cell culture supernatant