Human VEGF-C Quantikine ELISA Kit

R&D Systems | Catalog # DVEC00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (50 µL), Platelet-poor Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

48.4 pg/mL

Assay Range

109-7000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma)
View all VEGF-C ELISA Kits »

Product Summary for Human VEGF-C Quantikine ELISA Kit

The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, and plasma. It contains recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 572 1622 3243 520 1527 3069
Standard Deviation 39.3 54.4 136 50.1 102 200
CV% 6.9 3.4 4.2 9.6 6.7 6.5

EDTA Plasma, Heparin Plasma, Platelet-poor Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 512 1543 3195 514 1540 3066
Standard Deviation 33.8 54.2 136 43.6 110 198
CV% 6.6 3.5 4.3 8.5 7.2 6.4

Recovery for Human VEGF-C Quantikine ELISA Kit

The recovery of VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 107 93-115
EDTA Plasma (n=4) 101 96-110
Heparin Plasma (n=4) 97 88-103
Serum (n=4) 99 89-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of VEGF-C were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human VEGF-C ELISA Linearity

Scientific Data Images for Human VEGF-C Quantikine ELISA Kit

Human VEGF-C ELISA Cell Culture Supernate Standard Curve

Human VEGF-C ELISA Cell Culture Supernate Standard Curve

Human VEGF-C ELISA Serum/Plasma Standard Curve

Human VEGF-C ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Entrez Gene IDs

7424 (Human); 22341 (Mouse)

Gene Symbol

VEGFC

Additional VEGF-C Products

Product Documents for Human VEGF-C Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VEGF-C Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human VEGF-C Quantikine ELISA Kit

Customer Reviews for Human VEGF-C Quantikine ELISA Kit (3)

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  • Human VEGF-C Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HeLa cells
    Verified Customer | Posted 08/26/2020
  • Human VEGF-C Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human cell conditioned medium and Mouse cell conditioned medium
    Verified Customer | Posted 12/12/2019
    Works for mouse samples
    Human VEGF-C Quantikine ELISA Kit DVEC00
  • Human VEGF-C Quantikine ELISA Kit
    Name: Jenna Nguyen
    Sample Tested: 7TD1 mouse hybridoma cell line
    Verified Customer | Posted 10/17/2019

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Protocols

View specific protocols for Human VEGF-C Quantikine ELISA Kit (DVEC00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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