Human VEGF R3/Flt-4 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human VEGF R3/Flt‑4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25‑30% cross‑reactivity with recombinant mouse VEGF R3 is observed and no cross-reactivity with recombinant human (rh) VEGF R1 or rhVEGF R2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 54703
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human VEGF R3/Flt‑4
    Tyr25-Ile776
    Accession # P35916
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human VEGF R3/Flt-4 Fc Chimera (Catalog # 349-F4) under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human lung
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
VEGF R3/Flt‑4 in HUVEC Human Cells.

VEGF R3/Flt‑4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human VEGF R3/Flt‑4 Monoclonal Antibody (Catalog # MAB3491) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R3/Flt-4

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R3 cDNA encodes a 1298 amino acid (aa) precursor with a 24 aa signal peptide. Mature VEGF R3 is composed of a 751 aa extracellular domain, a 22 aa transmembrane domain and a 482 aa cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.

  • References:
    1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  • Long Name:
    Vascular Endothelial Growth Factor Receptor 3
  • Entrez Gene IDs:
    2324 (Human); 14257 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Heparins that block VEGF-A-mediated von Willebrand factor fiber generation are potent inhibitors of hematogenous but not lymphatic metastasis
    Authors: L Goertz, SW Schneider, A Desch, FT Mayer, J Koett, K Nowak, I Karampinis, MK Bohlmann, V Umansky, AT Bauer
    Oncotarget, 2016;7(42):68527-68545.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Further evidence for expression and function of the VEGF-C/VEGFR-3 axis in cancer cells.
    Authors: Su JL, Chen PS, Chien MH, Chen PB, Chen YH, Lai CC, Hung MC, Kuo ML
    Cancer Cell, 2008;13(6):557-60.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. The VEGF-C/Flt-4 axis promotes invasion and metastasis of cancer cells.
    Authors: Su JL, Yang PC, Shih JY, Yang CY, Wei LH, Hsieh CY, Chou CH, Jeng YM, Wang MY, Chang KJ, Hung MC, Kuo ML
    Cancer Cell, 2006;9(3):209-23.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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Isotype Controls
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