Human VEGFR3/Flt-4 Antibody Summary
Accession # P35916
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
VEGF R3/Flt‑4 in HUVEC Human Cells.
VEGF R3/Flt‑4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human VEGF R3/Flt‑4 Monoclonal Antibody (Catalog # MAB3491) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R3 cDNA encodes a 1298 amino acid (aa) precursor with a 24 aa signal peptide. Mature VEGF R3 is composed of a 751 aa extracellular domain, a 22 aa transmembrane domain and a 482 aa cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.
Citations for Human VEGFR3/Flt-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Correlation of Vascular Endothelial Growth Factor subtypes and their receptors with melanoma progression: A next-generation Tissue Microarray (ngTMA) automated analysis
Authors: SM Seyed Jafa, C Wiedmer, S Cazzaniga, Ž Frangež, M Shafighi, H Beltramine, B Weber, HU Simon, RE Hunger
PLoS ONE, 2018;13(11):e0207019.
Sample Types: Tissue Microarray
Heparins that block VEGF-A-mediated von Willebrand factor fiber generation are potent inhibitors of hematogenous but not lymphatic metastasis
Authors: L Goertz, SW Schneider, A Desch, FT Mayer, J Koett, K Nowak, I Karampinis, MK Bohlmann, V Umansky, AT Bauer
Sample Types: Whole Cells
Further evidence for expression and function of the VEGF-C/VEGFR-3 axis in cancer cells.
Authors: Su JL, Chen PS, Chien MH, Chen PB, Chen YH, Lai CC, Hung MC, Kuo ML
Cancer Cell, 2008;13(6):557-60.
Sample Types: Cell Lysates
Applications: Western Blot
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007;177(3):489-500.
Sample Types: Whole Cells
Applications: Flow Cytometry
The VEGF-C/Flt-4 axis promotes invasion and metastasis of cancer cells.
Authors: Su JL, Yang PC, Shih JY, Yang CY, Wei LH, Hsieh CY, Chou CH, Jeng YM, Wang MY, Chang KJ, Hung MC, Kuo ML
Cancer Cell, 2006;9(3):209-23.
Sample Types: Whole Tissue
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