Human VEGFR3/Flt-4 Antibody (54703)

R&D Systems | Catalog # MAB3491

R&D Systems
Discontinued Product
MAB3491 has been discontinued. View all VEGFR3/Flt-4 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 54703
View all VEGFR3/Flt-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VEGFR3/Flt‑4
Tyr25-Ile776
Accession # P35916

Specificity

Detects human VEGFR3/Flt‑4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25‑30% cross‑reactivity with recombinant mouse VEGFR3 is observed and no cross-reactivity with recombinant human (rh) VEGFR1 or rhVEGFR2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VEGFR3/Flt-4 Antibody (54703)

VEGFR3/Flt-4 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

VEGFR3/Flt‑4 in HUVEC Human Cells.

VEGFR3/Flt-4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human VEGFR3/Flt-4 Monoclonal Antibody (Catalog # MAB3491) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human VEGFR3/Flt-4 Antibody (54703)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung

Western Blot

1 µg/mL
Sample: Recombinant Human VEGFR3/Flt-4 Fc Chimera (Catalog # 349-F4) under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB3491 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR3/Flt-4

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR3 cDNA encodes a 1298 amino acid (aa) precursor with a 24 aa signal peptide. Mature VEGFR3 is composed of a 751 aa extracellular domain, a 22 aa transmembrane domain and a 482 aa cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt-4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

References

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Long Name

Vascular Endothelial Growth Factor Receptor 3

Alternate Names

Flt-4, FLT4, VEGF R3

Entrez Gene IDs

2324 (Human); 14257 (Mouse)

Gene Symbol

FLT4

UniProt

P35916

Additional VEGFR3/Flt-4 Products

Product Documents for Human VEGFR3/Flt-4 Antibody (54703)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VEGFR3/Flt-4 Antibody (54703)

For research use only

Citations for Human VEGFR3/Flt-4 Antibody (54703)

Customer Reviews for Human VEGFR3/Flt-4 Antibody (54703) (1)

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  • Human VEGFR3/Flt-4 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 11/02/2021
    Human VEGFR3/Flt-4 Antibody (54703) MAB3491

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