Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.
Human Versican Isoform V0 Biotinylated Antibody
R&D Systems | Catalog # BAF3054
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Versican Isoform V0
Gly1344-Asp1554
Accession # P13611
Gly1344-Asp1554
Accession # P13611
Specificity
Detects human Versican Isoform V0 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Versican Isoform V0 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Versican Isoform V0
Sample: Recombinant Human Versican Isoform V0
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Versican
Alternate Names
CSPG2, GHAP, PG-M, VCAN
Gene Symbol
VCAN
UniProt
Additional Versican Products
Product Documents for Human Versican Isoform V0 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Versican Isoform V0 Biotinylated Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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