Human Versican Isoform V0 Antibody [Biotin]

R&D Systems | Catalog # BAF3054

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human Versican Isoform V0
Gly1344-Asp1554
Accession # P13611

Specificity

Detects human Versican Isoform V0 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Versican Isoform V0 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Versican Isoform V0

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Versican

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Alternate Names

CSPG2, GHAP, PG-M, VCAN

Entrez Gene IDs

1462 (Human); 13003 (Mouse)

Gene Symbol

VCAN

UniProt

Additional Versican Products

Product Documents for Human Versican Isoform V0 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Versican Isoform V0 Antibody [Biotin]

For research use only

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