Human Versican Isoform V0 Antibody

Catalog # Availability Size / Price Qty
AF3054
AF3054-SP
Human Versican Isoform V0 Antibody in Immunocytochemistry
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Product Details
Citations (2)
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Human Versican Isoform V0 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Versican isoform V0 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Versican isoform V0
Gly1344-Asp1554
Accession # P13611
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Versican Isoform V0
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Versican in HeLa Human Cell Line. View Larger

Versican in HeLa Human Cell Line. Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Versican

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Entrez Gene IDs
1462 (Human); 13003 (Mouse)
Alternate Names
Chondroitin sulfate proteoglycan 2WGN; Chondroitin sulfate proteoglycan core protein 2; CSPG2; CSPG2DKFZp686K06110; ERVR; GHAP; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PG-MWGN1; VCAN; versican core protein; versican proteoglycan; Versican

Product Datasheets

Citations for Human Versican Isoform V0 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Versican is differentially regulated in the adventitial and medial layers of human vein grafts
    Authors: RD Kenagy, S Kikuchi, SP Evanko, MS Ruiter, M Piola, A Longchamp, M Pesce, M Soncini, S Deglise, GB Fiore, JA Haefliger, TA Schmidt, MW Majesky, M Sobel, TN Wight
    PLoS ONE, 2018;13(9):e0204045.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Versican overexpression in human breast cancer lesions: known and new isoforms for stromal tumor targeting.
    Authors: Kischel P, Waltregny D, Dumont B, Turtoi A, Greffe Y, Kirsch S, De Pauw E, Castronovo V
    Int. J. Cancer, 2010;126(3):640-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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