Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Versican Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Versican isoform V0
Gly1344-Asp1554
Accession # P13611

Specificity

Detects human Versican isoform V0 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Versican Isoform V0 Antibody

Versican antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Versican in HeLa Human Cell Line.

Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Human Versican Isoform V0 Antibody by Immunocytochemistry/ Immunofluorescence

Detection of Human Human Versican Isoform V0 Antibody by Immunocytochemistry/ Immunofluorescence

Analysis of the expression of specialized markers in the dermal spheroids: CD44 (green), alpha SMA (red); VERSICAN (green), Ki67 (red); VIMENTIN (green), FIBRONECTIN (red). Fluorescence microscopy, the scale length in all pictures is 100 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/36078136), licensed under a CC-BY license. Not internally tested by R&D Systems.

Versican in U‑251 MG Human Cell Line.

Versican was detected in immersion fixed U‑251 MG human glioblastoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Versican Isoform V0 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and U‑251 MG human glioblastoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Versican Isoform V0

Reviewed Applications

Read 1 review rated 4 using AF3054 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Versican

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Alternate Names

CSPG2, GHAP, PG-M, VCAN

Entrez Gene IDs

1462 (Human); 13003 (Mouse)

Gene Symbol

VCAN

UniProt

P13611

Additional Versican Products

Product Documents for Human Versican Isoform V0 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Versican Isoform V0 Antibody

For research use only

Citations for Human Versican Isoform V0 Antibody

Customer Reviews for Human Versican Isoform V0 Antibody (1)

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Customer Images

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  • Human Versican Isoform V0 Antibody
    Name: Shrunal Mane
    Application: Immunohistochemistry
    Sample Tested: Skin cancer tissue
    Species: Human
    Verified Customer | Posted 11/11/2024
    FFPE section of cSCC tumour tissue stained with VCAN (AF3054) in brown. HIER of pH 6 was used. Primary antibody dilution of 1:200 was used and secondary anti-Goat HRP was used for detection. Blue staining represt nuclei.
    Human Versican Isoform V0 Antibody AF3054

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Protocols

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