Human Versican Isoform V0 Antibody Summary
Accession # P13611
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Versican in HeLa Human Cell Line. Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.
Citations for Human Versican Isoform V0 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Versican is differentially regulated in the adventitial and medial layers of human vein grafts
Authors: RD Kenagy, S Kikuchi, SP Evanko, MS Ruiter, M Piola, A Longchamp, M Pesce, M Soncini, S Deglise, GB Fiore, JA Haefliger, TA Schmidt, MW Majesky, M Sobel, TN Wight
PLoS ONE, 2018;13(9):e0204045.
Sample Types: Tissue Homogenates
Applications: Western Blot
Versican overexpression in human breast cancer lesions: known and new isoforms for stromal tumor targeting.
Authors: Kischel P, Waltregny D, Dumont B, Turtoi A, Greffe Y, Kirsch S, De Pauw E, Castronovo V
Int. J. Cancer, 2010;126(3):640-50.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human Versican Isoform V0 Antibody
There are currently no reviews for this product. Be the first to review Human Versican Isoform V0 Antibody and earn rewards!
Have you used Human Versican Isoform V0 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image