Human VIAAT/SLC32A1 Antibody

AF6847 Datasheet / COA / SDS

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AF6847
AF6847-SP

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Human VIAAT/SLC32A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VIAAT/SLC32A1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human VIAAT/SLC32A1
Ala2-Phe133
Accession # Q9H598

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human VIAAT/SLC32A1 by Western Blot. Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human VIAAT/SLC32A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6847) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VIAAT/SLC32A1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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VIAAT/SLC32A1 in Human Brain. VIAAT/SLC32A1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate putamen) using Sheep Anti-Human VIAAT/SLC32A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6847) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIAAT/SLC32A1

VIAAT (Vesicular inhibitory amino acid transporter; also VGAT and SLC32A1) is a 56-58 kDa member of the amino acid/polyamine transporter 2 family of proteins. It is expressed in inhibitory neurons throughout the CNS, in both resting and activated lymphocytes, and in pancreatic alpha -cells (in rodent). VIAAT is believed to transport both GABA and glycine across synaptic vesicle membranes in an electrical and pH gradient-dependent manner. Once transported, these neurotransmitters are released at the presynaptic membrane, acting as inhibitory factors in the mature nervous system, and excitatory factors in the immature nervous system. Human VIAAT is a 525 amino acid (aa) 10-transmembrane nonglycosylated protein. The N-terminus (aa 1-133) and C-terminus (aa 511-525) are cytoplasmic. There is a 52 kDa short form that is not well characterized. Over aa 2-133, human VIAAT shares 95% aa identity with mouse VIAAT.

Long Name

Vesicular Inhibitory Amino Acid Transporter

Entrez Gene IDs

140679 (Human); 22348 (Mouse); 83612 (Rat)

Alternate Names

bA122O1.1; hVIAAT; SLC32A1; solute carrier family 32 (GABA vesicular transporter), member 1; Solute carrier family 32 member 1; Vesicular GABA transporter; vesicular inhibitory amino acid transporter; VGAT; VGATGABA and glycine transporter; VIAAT