|Detection of VIP R2 in HEK293 Human Cell Line Transfected with Human VIP R2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human VIP R2 and eGFP were stained with either (A) Mouse Anti-Human VIP R2 PE‑conjugated Monoclonal Antibody (Catalog # FAB5416P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.|
Human VIP R2 (Vasoactive Intestinal Polypeptide Receptor 2), also known as VPAC2 is a 60-70 kDa seven transmembrane glycoprotein receptor that belongs to the G protein coupled receptor 2 family of proteins. The mature chain contains in its first extracellular region a hormone receptor domain (aa 49‑114) which includes three N-linked glycosylation sites and a "cilia targeting signal" comprised of and Arg-Asp-Tyr-Arg sequence. Human VIP R2 shares 85% aa sequence identity with mouse and rat VIP R2. In the extracellular domains, human VIP R2 shares 88% and 31% aa sequence identity with mouse VIP R2 and human VIP R1, respectively. VIP R2 is expressed predominantly in skeletal muscle, CD4+ T cells, smooth muscle cells, plasmacytoid dendritic cells, hepatic progenitor cells, monocytes, synoviocytes and select neurons. VIP R1 and VIP R2 bind VIP with equal affinity, but the two receptors are not redundant. VIP R1 interacts with RAMPs; VIP R2 does not. In the dentate gyrus, VIP R1 drives stem cells into a granule cell phenotype while VIP R2 maintains a Nestin+ phenotype.