Human VSIG2 Antibody

Catalog # Availability Size / Price Qty
MAB51771-100
MAB51771-SP
Detection of VSIG2 in HEK293 Human Cell Line  Transfected with Human VSIG2  and eGFP by Flow Cytometry.
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Human VSIG2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VSIG2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 541522
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG2
Val24-Ala243
Accession # Q961Q7-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of VSIG2 in HEK293 Human Cell Line  Transfected with Human VSIG2  and eGFP by Flow Cytometry. View Larger

Detection of VSIG2 in HEK293 Human Cell Line Transfected with Human VSIG2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human VSIG2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG2 Monoclonal Antibody (Catalog # MAB51771) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG2

V-set and immunoglobulin domain-containing protein 2 (VSIG2), also known as cortical thymocyte-like protein (CT-like protein), is a member of the immunoglobulin superfamily (Igsf) that was originally described in Xenopus and was referred to as cortical thymocyte marker in Xenopus, or CTX. The clone isolated from the human genomic library was called CTH (1). Mature human VSIG2 is a single-pass type I transmembrane protein with a 220 amino acid (aa) extracellular domain (ECD) that contains one C2-type and one V-type immunoglobulin-like domains, and a 63 aa cytoplasmic region linked to the ECD by a short 21 aa transmembrane domain. Human VSIG2 ECD shares 85% and 86% aa sequence identity with mouse and rat VSIG2, respectively. VSIG2 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG2 inhibits T cell activation, including IL17 and interferon gamma production.

References
  1. Chrétien, I. et al. (1998) Eur. J. Immunol. 28:4094.
Long Name
V-Set and Immunoglobulin Domain Containing 2
Entrez Gene IDs
23584 (Human); 57276 (Mouse); 300520 (Rat)
Alternate Names
2210413P10Rik; Cortical thymocyte-like protein; CTH; CTHCTXLcortical thymocyte receptor (X. laevis CTX) like; CT-like protein; CTXL; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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