Human vWF-A2 APC-conjugated Antibody
Human vWF-A2 APC-conjugated Antibody Summary
Asp1498-Val1665
Accession # P04275
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of vWF‑A2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human vWF-A2 APC-conjugated Mono-clonal Antibody (Catalog # IC27641A, filled histo-gram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: vWF-A2
von Willebrand Factor (vWF) is a large, multimeric glycoprotein made by endothelial cells and megakaryocytes. The pre-pro-vWF protein contains 2813 amino acids (aa), which consists of a 22 aa signal peptide, a 741 aa propeptide and a mature vWF monomer of 2050 aa (1-4). The pro-vWF undergoes dimerization in the endoplasmic reticulum (ER) through a C-terminal “cysteine-knot” (CK) domain. The pro-vWF dimers are transported to the Golgi and associate into multimers by forming disulfide bonds in the amino-terminal region of the mature form. Proteolytic processing of the pro-region also occurs in the Golgi. Mature vWF is stored in Weibel-Pallade bodies in endothelial cells and granules in megakaryocytes and platelets. The unusually-large vWF (ulvWF) multimers released from cells are very efficient in binding to platelets to form thrombus. The population of these highly active ulvWF multimers is controlled by a specific protease, ADAMTS13, which cleaves between residues Tyr1605 and Met1606 in the A2 domain of vWF. In the plasma, vWF appears as a series of large and intermediate size multimers with molecular masses from several thousand to 500 kDa. vWF also performs hemostatic functions (3-5). In a high shear-stressed environment, vWF undergoes conformational changes to expose a binding site for glycoprotein Ib alpha. As a result, vWF facilitates the aggregation of platelets. In addition to platelet binding, vWF binds coagulation factor VIII to increase the lifetime of FVIII in plasma. The purified rhvWF-A2 contains the A2 domain of vWF.
- Sadler, J. E. (1998) Annu. Rev. Biochem. 67:395.
- Ruggeri, Z. M. (2003) Cur. Opin. Hemat. 10:142.
- Michiels, J. J. et al. (2006) Clin. Appl. Thromb. Hemost. 12:397.
- Groot, E. et al. (2007) Cur. Opin. Hemat. 14:284.
- Lenting, P. J. et al. (2007) J. Thromb. Haemos. 5:1353.
Product Datasheets
Citation for Human vWF-A2 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Proteasome proteolysis supports stimulated platelet function and thrombosis.
Authors: Gupta N, Li W, Willard B, Silverstein R, McIntyre T
Arterioscler Thromb Vasc Biol, 2013-10-31;34(1):160-8.
Species: Human
Sample Types: Whole Blood
Applications: Flow Cytometry
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