Human WIF-1 Biotinylated Antibody

Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Product Details
Citations (1)
Supplemental Products

Human WIF-1 Biotinylated Antibody Summary

Species Reactivity
Detects human WIF-1 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with recombinant mouse WIF-1 or recombinant human Wnt-7a is observed. In Western blots, 50% cross-reactivity with recombinant mouse WIF-1 is observed.
Monoclonal Mouse IgG1 Clone # 133016
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human WIF-1
Accession # AAD25402
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human WIF‑1 (Catalog # 1341-WF)

Human WIF-1 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human WIF‑1 Antibody (Catalog # MAB1342)

Standard: Recombinant Human WIF-1 Protein (Catalog # 1341-WF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WIF-1

WIF (Wnt Inhibitory Factor) is a secreted protein that binds to Wnt proteins and inhibits their activity. The protein is synthesized as a 379 amino acid (aa) molecule that contains an N-terminal signal sequence, 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus (1). In situ hybridization analysis from the frog, Xenopus laevis, and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles (1). WIF inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos (1). In vitro, WIF binds to Drosophila Wingless and Xenopus Wnt‑8 proteins (1). WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung and bladder (2). However, WIF-1’s role in carcinogenesis may not be that simple since in other cancer types, such as colon adenocarcinoma, WIF facilitates tumorigenesis (3). Human WIF-1 shares 94% and 82% amino acid identity with mouse and frog, respectively.

  1. Hsieh, J-C. et al. (1999) Nature 398:431.
  2. Wissmann, C. et al. (2003) J. Pathol 201:204.
  3. Cebrat, M. et al. (2004) Cancer Lett. 206:107.
Long Name
Wnt Inhibitory Factor
Entrez Gene IDs
11197 (Human); 24117 (Mouse)
Alternate Names
WIF1; WIF-1; WNT inhibitory factor 1

Product Datasheets

You must select a language.


Citation for Human WIF-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Role of Wnt/ beta -catenin and RANKL/OPG in bone healing of diabetic Charcot arthropathy patients
    Authors: Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al.
    Acta Orthopaedica


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human WIF-1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human WIF-1 Biotinylated Antibody and earn rewards!

Have you used Human WIF-1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review