Human Wnt-3a and Inhibitor IWR Set Protein Summary
Product Specifications
Recombinant Human Wnt-3a induces beta -catenin responsive Topflash activation in HEK293T cells. The specific Wnt pathway inhibitor, endo-IWR 1, inhibits Topflash activity from a 2 μg/mL dose of Recombinant Human Wnt-3a with a typical ED50 < 0.2 μM. The less active diasteriomer exo-IWR 1 Wnt inhibitor shows minor inhibitory activity in this assay.
Recombinant Human Wnt-3a induces alkaline phosphatase production in the MC3T3-E1 mouse pre-osteoblast cell line. The ED50 for this effect is 5-25 ng/mL. The specific Wnt pathway inhibitor, endo-IWR 1, inhibits alkaline phosphatase production induced from a 50 ng/mL dose of Recombinant Human Wnt-3a with an typical ED50 < 0.8 μM. The less active diasteriomer exo-IWR 1 Wnt inhibitor shows a > 5 μM ED50 with a Recombinant Human Wnt-3a dose of 50 ng/ml.
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
PSM5036
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: Wnt-3a
Wnt-3a is a secreted glycoprotein that is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (1-4). Wnt-3a also promotes self-renewal of hematopoietic stem cells (5,6). Wnt-3a, one of 19 vertebrate members of the Wnt family, binds to Frizzled receptor proteins along with LRP5/6 transmembrane proteins causing an intracellular cascade ultimately resulting in increased intracellular beta -catenin levels (7). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to Wnt/ beta -catenin responsive gene activation (8). The intracellular protein Axin is a critical scaffold protein necessary for assembly of the beta -catenin destruction complex that consists of APC, GSK-3 beta, and beta -catenin. Once bound to this complex, beta -catenin is phosphorylated and targeted for degradation (9). Endo-IWR 1 is a small molecule inhibitor of Axin turnover resulting in stabilization of the beta -catenin destruction complex and suppression of Wnt signal transduction. The diastereomeric form of IWR 1, exo-IWR 1, exhibits decreased Axin stabilizing activity compared to endo-IWR 1 and can be used as a specificity control (10).
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