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Human Wnt-6 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF4109T Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4109T-100UG

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Human Wnt-6 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Wnt-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Wnt-4, rmWnt-8a, and rmWnt-9b is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Wnt‑6
Met35-Glu75 and Gln238-Arg299
Accession # Q9Y6F9
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Wnt-6

Human Wnt-6 is a 46 kDa, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells, and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse, and canine Wnt-6 are identical.

Long Name
Wingless-type MMTV Integration Site Family, Member 6
Entrez Gene IDs
7475 (Human); 22420 (Mouse); 316526 (Rat)
Alternate Names
protein Wnt-6; wingless-type MMTV integration site family, member 6; Wnt6; Wnt-6

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