Human Wnt-7a/b Antibody

Catalog # Availability Size / Price Qty
AF3008
AF3008-SP
Wnt‑7a/b in Human endometrial Cancer Tissue.
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Product Details
Citations (4)
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Human Wnt-7a/b Antibody Summary

Species Reactivity
Human
Specificity
Detects human Wnt‑7a and Wnt-7b in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt‑16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Wnt‑7a
Leu32-Glu72, Thr216-Arg292
Accession # O00755
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Wnt‑7a (Catalog # 3008-WN) and Recombinat Human Wnt-7b
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Wnt‑7a/b in Human endometrial Cancer Tissue. View Larger

Wnt‑7a/b in Human endometrial Cancer Tissue.

Wnt‑7a/b was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using 15 µg/mL Goat Anti-Human
Wnt‑7a/b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3008) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-7a/b

Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor‑related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta ‑catenin-mediated gene expression (1, 2). Human Wnt‑7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain (3, 4). Palmitate modification of a cysteine residue has been shown for Wnt‑3a; this site is conserved on all Wnts and is Cys73 on Wnt‑7a (5). When modified, increased hydrophobicity and activity is expected. Human Wnt‑7a shows 97% aa identity with mouse, rat, and dog Wnt‑7a and 92% aa identity with chicken Wnt‑7a. During development, Wnt‑7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme (6-10). When Wnt‑7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract (6-10). Wnt‑7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control (11, 12). Roles for Wnt‑7a have also been shown during formation of neural synapses, response of the uterus to estrogen and inflammatory cartilage destruction (10, 13, 14). Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Wnt-7b is a ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a.

References
  1. Logan, C.Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  2. Wong, G. T. et al. (1994) Mol. Cell. Biol. 14:6278.
  3. http://www.stanford.edu/~rnusse/wntwindow.html 
  4. Bui, T. D. et al. (1997) Gene 189:25.
  5. Willert, K. et al. (2003) Nature 423:448.
  6. Parr, B.A. and A.P. McMahon (1995) Nature 374:350.
  7. Cygan, J.A. et al. (1997) Development 124:5021.
  8. Parr, B.A. and A.P. McMahon (1998) Nature 395:707.
  9. Miller, C. and D. Sassoon (1998) Development 125:3201.
  10. Carta, L. and D. Sassoon (2004) Biol. Reprod. 71:444.
  11. Calvo, R. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12776.
  12. Winn, R.A. et al. (2005) J. Biol Chem. 280:19625.
  13. Hall, A.C. et al. (2000) Cell 100:525.
  14. Hwang, S-G. et al. (2004) J. Biol. Chem 279:26597.
Long Name
Wingless-type MMTV Integration Site Family, Member 7a/b (Cross-reactive)
Alternate Names
Wnt-7a/b

Product Datasheets

Citations for Human Wnt-7a/b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes
    Authors: SH Lee, SM Shin, P Zhong, HT Kim, DI Kim, JM Kim, W Do Heo, DW Kim, CY Yeo, CH Kim, QS Liu
    Nat Commun, 2018;9(1):3434.
    Species: Rat
    Sample Types: Exosomes
    Applications: WB
  2. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
    Authors: Bruce F Kimler
    J. Endocrinol., 2016;229(2):197-207.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells.
    Authors: Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K
    Int J Oncol, 2012;41(6):2021-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
    Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
    Neuron, 2009;62(4):510-25.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

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