Detection of XCR1 in CD141+ HLA-DR+ Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with (A) Mouse Anti-Human HLA-DR Phycoerythrin-Conjugated MonoclonalAntibody (Catalog # FAB4869P), Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog #FAB3947A) and (B) Rabbit Anti-Human XCR1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB8571G, filled histogram) or isotype control antibody (Catalog # IC105G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 degreesC as supplied.
XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8 + T cells, NK cells, CD4 + T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.
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