Human XCR1 PE-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8571P-025
FAB8571P-100
Detection  of XCR1 in CD141+ HLA-DR+ Human Blood Monocytes by Flow Cytometry.
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Human XCR1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human XCR1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1097A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of XCR1 in CD141+ HLA-DR+ Human Blood Monocytes by Flow Cytometry. View Larger

Detection of XCR1 in CD141+ HLA-DR+ Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated MonoclonalAntibody (Catalog # FAB4869F), Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog #FAB3947A) and (B) Rabbit Anti-Human XCR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB8571P, filled histogram) or isotype control antibody (Catalog # IC105P, open histogram). View our protocol for Staining Membrane-associated Proteins

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: XCR1

XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Long Name
Chemokine [C Motif] Receptor 1
Entrez Gene IDs
2829 (Human); 23832 (Mouse); 301086 (Rat)
Alternate Names
CCXCR1; CCXCR1XC chemokine receptor 1; chemokine (C motif) receptor 1; chemokine XC receptor 1; G protein-coupled receptor 5; GPR5; GPR5chemokine (C motif) XC receptor 1; G-protein coupled receptor 5; Lymphotactin receptor; XCR1

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Isotype Controls

Reviews for Human XCR1 PE-conjugated Antibody

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Human XCR1 PE-conjugated Antibody
By Anonymous on 06/24/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

this antibody was used to stain BDCA3 defined by XCR1+BDCA3+ dendritic cells and it works very well.