Detection of XCR1 in CD141+ and HLA-DR+ Human Blood Monocytes by Flow Cytometry.
CD141+ and HLA-DR+ human peripheral blood monocytes were isolated by staining with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated MonoclonalAntibody (Catalog # FAB4869F)and Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog #FAB3947A). Isolated cells were then stained with (B) RabbitAnti-Human XCR1 Monoclonal Antibody (Catalog # MAB8571, filled histogram) orisotype control antibody (Catalog # AB-105-C,open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG SecondaryAntibody (Catalog # F0110).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.
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