Human XCR1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8571
MAB8571-SP
Detection  of XCR1 in CD141+ and HLA-DR+ Human Blood Monocytes by Flow Cytometry.
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Human XCR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human XCR1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1097A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of XCR1 in CD141+ and HLA-DR+ Human Blood Monocytes by Flow Cytometry. View Larger

Detection of XCR1 in CD141+ and HLA-DR+ Human Blood Monocytes by Flow Cytometry. CD141+ and HLA-DR+ human peripheral blood monocytes were isolated by staining with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated MonoclonalAntibody (Catalog # FAB4869F)and Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog #FAB3947A).  Isolated cells were then stained with (B) RabbitAnti-Human XCR1 Monoclonal Antibody
(Catalog # MAB8571, filled histogram) orisotype control antibody (Catalog # AB-105-C,open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG SecondaryAntibody (Catalog # F0110).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCR1

XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Long Name
Chemokine [C Motif] Receptor 1
Entrez Gene IDs
2829 (Human); 23832 (Mouse); 301086 (Rat)
Alternate Names
CCXCR1; CCXCR1XC chemokine receptor 1; chemokine (C motif) receptor 1; chemokine XC receptor 1; G protein-coupled receptor 5; GPR5; GPR5chemokine (C motif) XC receptor 1; G-protein coupled receptor 5; Lymphotactin receptor; XCR1

Product Datasheets

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