Detection of Human XPE/DDB2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPE/DDB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPE/DDB2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Xeroderma Pigmentosum complementation group E (XPE) patients are defective in a heterodimer of damage-specific DNA-binding activity (DDB1/DDB2). DDB2 is a 48 kDa protein upregulated in cells exposed to ultraviolet light. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.
Xeroderma Pigmentosum E
Entrez Gene IDs:
1643 (Human); 107986 (Mouse); 311186 (Rat)
damage-specific DNA binding protein 2 (48kD); damage-specific DNA binding protein 2, 48kDa; Damage-specific DNA-binding protein 2; DDB p48 subunit; DDB2; DDBB; DNA damage-binding protein 2; FLJ34321; UV-damaged DNA-binding protein 2; UV-DDB 2; UV-DDB2; xeroderma pigmentosum group E protein; XP5
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