Human ZBP1/DLM-1/DAI Antibody

Catalog # Availability Size / Price Qty
Detection of Human ZBP1/DLM‑1/DAI by Western Blot.
1 Image
Product Details
Citations (2)
Supplemental Products

Human ZBP1/DLM-1/DAI Antibody Summary

Species Reactivity
Detects human ZBP1/DLM-1/DAI in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human ZBP1/DLM-1/DAI
Accession # Q9H171
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ZBP1/DLM-1/DAI antibody by Western Blot. View Larger

Detection of Human ZBP1/DLM‑1/DAI by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL) and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZBP1/DLM-1/DAI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6309) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ZBP1/DLM-1/DAI at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZBP1/DLM-1/DAI

ZBP1 (Z-DNA binding protein 1; also DAI and DLM-1) is a 15-60 kDa cytosolic detector of intracellular DNA. In a TOLL-independent manner, ZBP1 detects cytoplasmic dsDNA and initiates INF-beta production. It does so by binding DNA, followed by multimerization and recruitment of TBK1 and IRF3. This is accompanied by a parallel interaction with RIP-1 and -3 that stimulates NF kappa B activity and cytokine production. Human ZBP1 is 429 amino acids (aa) in length. It contains two DNA-binding Z-regions (aa 6-72 and 103-168) plus a third DNA-binding region that likely lies in the region between aa 170-250. Multiple splice forms exist. One shows a deletion of aa 12-86, a second shows a 63 aa substitution for aa 87-429, a third contains a 24 aa substitution for aa 225-429, a fourth reveals a deletion of aa 87-109, while a fifth and sixth show 82 and 16 aa substitutions for aa 365-429, respectively. Full length human ZBP1 shares only 47% aa identity with mouse ZBP1.

Long Name
Zipcode-binding Protein 1
Entrez Gene IDs
81030 (Human); 58203 (Mouse); 171091 (Rat)
Alternate Names
C20orf183; chromosome 20 open reading frame 183; DAI; DLM1; DLM-1; DLM1dJ718J7.3; DNA-dependent activator of interferon regulatory factors; DNA-dependent activator of IRFs; Tumor stroma and activated macrophage protein DLM-1; ZBP1 Z-DNA binding protein 1; ZBP1; Z-DNA binding protein 1; Z-DNA-binding protein 1

Product Datasheets

You must select a language.


Citations for Human ZBP1/DLM-1/DAI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
    Authors: R Cheng, X Liu, Z Wang, K Tang
    Molecular Medicine Reports, 2021;23(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation
  2. ZBP1 not RIPK1 mediates tumor necroptosis in breast cancer
    Authors: JY Baik, Z Liu, D Jiao, HJ Kwon, J Yan, C Kadigamuwa, M Choe, R Lake, M Kruhlak, M Tandon, Z Cai, S Choksi, ZG Liu
    Nature Communications, 2021;12(1):2666.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human ZBP1/DLM-1/DAI Antibody

There are currently no reviews for this product. Be the first to review Human ZBP1/DLM-1/DAI Antibody and earn rewards!

Have you used Human ZBP1/DLM-1/DAI Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review