Detection of Human ZBP1/DLM‑1/DAI by Western Blot.
Western blot shows lysates of human peripheral blood lymphocytes (PBL) and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZBP1/DLM‑1/DAI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6309) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ZBP1/DLM‑1/DAI at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ZBP1 (Z-DNA binding protein 1; also DAI and DLM-1) is a 15-60 kDa cytosolic detector of intracellular DNA. In a TOLL-independent manner, ZBP1 detects cytoplasmic dsDNA and initiates INF-beta production. It does so by binding DNA, followed by multimerization and recruitment of TBK1 and IRF3. This is accompanied by a parallel interaction with RIP-1 and -3 that stimulates NF kappa B activity and cytokine production. Human ZBP1 is 429 amino acids (aa) in length. It contains two DNA-binding Z-regions (aa 6-72 and 103-168) plus a third DNA-binding region that likely lies in the region between aa 170-250. Multiple splice forms exist. One shows a deletion of aa 12-86, a second shows a 63 aa substitution for aa 87-429, a third contains a 24 aa substitution for aa 225-429, a fourth reveals a deletion of aa 87-109, while a fifth and sixth show 82 and 16 aa substitutions for aa 365-429, respectively. Full length human ZBP1 shares only 47% aa identity with mouse ZBP1.
Zipcode-binding Protein 1
Entrez Gene IDs:
81030 (Human); 58203 (Mouse); 171091 (Rat)
C20orf183; chromosome 20 open reading frame 183; DAI; DLM1; DLM-1; DLM1dJ718J7.3; DNA-dependent activator of interferon regulatory factors; DNA-dependent activator of IRFs; Tumor stroma and activated macrophage protein DLM-1; ZBP1 Z-DNA binding protein 1; ZBP1; Z-DNA binding protein 1; Z-DNA-binding protein 1
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