Human ZMIZ1/Zimp10 Antibody

Catalog # Availability Size / Price Qty
Detection of Human ZMIZ1/Zimp10 by Western Blot.
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Product Details
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Human ZMIZ1/Zimp10 Antibody Summary

Species Reactivity
Detects human ZMIZ1/Zimp10 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human ZMIZ1/Zimp10
Accession # Q9ULJ6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ZMIZ1/Zimp10 antibody by Western Blot. View Larger

Detection of Human ZMIZ1/Zimp10 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZMIZ1/Zimp10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8107) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZMIZ1/Zimp10 at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ZMIZ1/Zimp10 antibody in Human Ovary by Immunohistochemistry (IHC-P). View Larger

ZMIZ1/Zimp10 in Human Ovary. ZMIZ1/Zimp10 was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human ZMIZ1/Zimp10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8107) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZMIZ1/Zimp10

Human ZMIZ1 (zinc finger and Miz-domain containing 1), previously called Zimp10 (zinc finger, Miz1, PIAS-like protein on chromosome 10) is a 1067 amino acid (aa), approximately 130 kDa cytoplasmic and nuclear protein. It is a coactivator of the androgen receptor (AR), and enhances AR-mediated transcription and AR sumoylation. It also coactivates other transcription factors such as SMAD4 and p53. It is normally expressed in human ovary, testis and prostate epithelial cells. The ZIMZ1 gene can be fused to ABL1 in a B-cell acute lymphocytic leukemia. Within the region used as an immunogen, human ZMIZ1 shares 98% and 97% aa sequence identity with mouse and rat ZMIZ1, respectively. A 997 aa isoform with an alternate sequence replacing aa 1-94 has been described.

Long Name
Zinc Finger, MIZ-Type Containing 1
Entrez Gene IDs
57178 (Human)
Alternate Names
FLJ13541; hZIMP10; KIAA1224; KIAA1224Zimp10; MIZ; PIAS-like protein Zimp10; RAI17; RAI17TRAFIP10; retinoic acid induced 17; Retinoic acid-induced protein 17; RP11-519K18.1; TRAFIP10; Zimp10; ZIMP10FLJ00092 protein; zinc finger MIZ domain-containing protein 1; zinc finger, MIZ-type containing 1; zinc finger-containing, Miz1, PIAS-like protein on chromosome 10; ZMIZ1

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