Huntingtin Antibody (HDB4E10) - BSA Free

Novus Biologicals | Catalog # NB600-1197

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HDB4E10

Format

BSA Free
View all Huntingtin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin

Reactivity Notes

Predicted cross-reactivities: Human, Mouse, Rabbit

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

This reacts with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Huntingtin Antibody (HDB4E10) - BSA Free

Western Blot: Huntingtin Antibody (HDB4E10)BSA Free [NB600-1197]

Western Blot: Huntingtin Antibody (HDB4E10)BSA Free [NB600-1197]

Western Blot: Huntingtin Antibody (HDB4E10) [NB600-1197] - Western Blot analysis of normal human cerebral cortex total protein extract run on a 3-12.5% gradient SDS-PAGE gel and probed with Mouse anti Huntingtin

Applications for Huntingtin Antibody (HDB4E10) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Immunohistology on frozen (paraffin not tested). Western blotting detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. This clone recognizes both denatured and native huntingtin in human brain.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Huntingtin

The combined use of clones HDC8A4, HDB4E10 and HDA3E10 demonstrate that huntingtin is enriched in neuronal cells in the brain.

Long Name

Huntingtin

Alternate Names

HD, HTT, IT15

Entrez Gene IDs

3064 (Human); 15194 (Mouse); 29424 (Rat)

Gene Symbol

HTT

UniProt

P42858 (Human)

Additional Huntingtin Products

Product Documents for Huntingtin Antibody (HDB4E10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Huntingtin Antibody (HDB4E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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