Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Inhibition Activity, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # 3E2B

Format

Azide and BSA Free
View all ICAM-1/CD54 Primary Antibodies »

Product Specifications

Immunogen

Mouse ICAM-1 (CD54)

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Western Blot: ICAM-1/CD54 Antibody (3E2B)Azide and BSA Free [NBP2-22540]

Western Blot: ICAM-1/CD54 Antibody (3E2B)Azide and BSA Free [NBP2-22540]

Western Blot: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of 25 ug of NIH-3T3 (lane 1), mouse spleen (lane 2) and Ramos (lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of CD54 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Normal biopsies of deparaffinized Mouse spleen tissue.
Flow Cytometry: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Flow Cytometry: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Flow Cytometry: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of CD54 in mouse splenocytes (green) compared to an isotype control (blue).
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Normal biopsies of deparaffinized Mouse kidney tissue.
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Normal biopsies of deparaffinized Mouse lung tissue.

Applications for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:10 - 1:100

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

15894 (Mouse)

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Customer Reviews for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free and earn rewards!

Have you used ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Could you please tell me whether the cat# NBP2-22540 can be used for IF.

    A: Our product NBP2-22540 has been validated in Flow and IHC in paraffin-embedded tissues. We have not yet tested the antibody in ICC. It is likely that this product would work in ICC as it has been found to work in other similar applications. We can also perform custom conjugations if you are interested in a directly conjugated antibody.

  • Q: Could you please tell me whether the cat# NBP2-22540 can be used for IF.

    A: Our ICAM1 antibody with catalogue number NBP2-22540 has not been validated for immunofluorescence with cells. It has however been tested for blocking/neutralising experiments, ELISA, flow cytometry and immunohistochemistry with paraffin-embedded tissue samples from mouse. It has not been tested with samples from any other species than mouse.

  • Q: Could you please tell me whether the cat# NBP2-22540 can be used for IF.

    A: Our product NBP2-22540 has been validated in Flow and IHC in paraffin-embedded tissues. We have not yet tested the antibody in ICC. It is likely that this product would work in ICC as it has been found to work in other similar applications. We can also perform custom conjugations if you are interested in a directly conjugated antibody.

  • Q: Could you please tell me whether the cat# NBP2-22540 can be used for IF.

    A: Our ICAM1 antibody with catalogue number NBP2-22540 has not been validated for immunofluorescence with cells. It has however been tested for blocking/neutralising experiments, ELISA, flow cytometry and immunohistochemistry with paraffin-embedded tissue samples from mouse. It has not been tested with samples from any other species than mouse.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies