ICOS Antibody

Novus Biologicals | Catalog # NBP1-52139

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ICOS Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KGGQILCDLTKTKGS corresponding to internal region according to NP_036224.1.

Epitope

KGGQILCDLTKTKGS

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ICOS Antibody

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining
Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139]

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ICOS Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 13 kDa band in human peripheral blood Lymphocyte lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6 kDa band according to NP_036224.1. The 13 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Lymph Node shows membrane staining just outside the mantle zone of the Germinal Centre.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ICOS

CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Long Name

Inducible Co-stimulator

Alternate Names

AILIM, CD278, CRP-1

Entrez Gene IDs

29851 (Human); 54167 (Mouse)

Gene Symbol

ICOS

UniProt

NP_036224.1 (Human)

Additional ICOS Products

Product Documents for ICOS Antibody

Certificate of Analysis

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Product Specific Notices for ICOS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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