ID1 Antibody (2C7) - BSA Free

Novus Biologicals | Catalog # NBP3-26211

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2C7 expressed in HEK293

Format

BSA Free
View all ID1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human ID1 [UniProt P41134]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ID1 Antibody (2C7) - BSA Free

ID1 Antibody (2C7)

Western Blot: ID1 Antibody (2C7) [NBP3-26211] -

Western Blot: ID1 Antibody (2C7) [NBP3-26211] - Positive Western Blot detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate. 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate.
All lanes: ID1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 17, 16 kDa
Observed band size: 26 kDa
ID1 Antibody (2C7)

Immunocytochemistry/Immunofluorescence: ID1 Antibody (2C7) [NBP3-26211] -

Immunocytochemistry/Immunofluorescence: ID1 Antibody (2C7) [NBP3-26211] - Staining of Hela Cells with ID1 Antibody (2C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for ID1 Antibody (2C7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ID1

Id1 is encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Inhibitor of DNA Binding 1

Alternate Names

BHLHB24, bHLHb24DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, dJ857M17.1.2, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein), ID, inhibitor of differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Gene Symbol

ID1

Additional ID1 Products

Product Documents for ID1 Antibody (2C7) - BSA Free

Certificate of Analysis

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Product Specific Notices for ID1 Antibody (2C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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