Catalog Number: 4016
Chemical Name: Heptanedioic acid-1-(2-cyclopentylidenehydrazide)
Biological Activity
Cell-permeable inducer of definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC50 = 223 nM for induction of Sox17 expression in ESCs). Reported to activate TGF-β signaling and downstream Smad2 phosphorylation; upregulates Nodal expression. Promotes pancreatic progenitor cell formation in vitro, and gut tube formation in vivo.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 20 mM in water and to 100 mM in DMSO
  • Purity:
  • Storage:
    Store at +4°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Licensing Caveats:
Sold for research purpose under license from Harvard University.
Background References
  1. Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells.
    Borowiak M, Maehr R, Chen S et al.
    Cell Stem Cell.
  2. Using small molecules to great effect in stem cell differentiation.
    Cell Stem Cell, 2009;4:373
  3. Stimulation of activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells.
    Filby et al.
    Stem Cell Res.Ther., 2011;2:16
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