IDO2 Antibody (1HC) - BSA Free

Novus Biologicals | Catalog # NBP2-21641

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1HC

Format

BSA Free
View all IDO2 Primary Antibodies »

Product Specifications

Immunogen

IDO2 Antibody (1HC) was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development. (Uniprot: Q8R0V5)

Reactivity Notes

Cross-reactivity with IDO2 from other sources has not been determined

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

This antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for IDO2 Antibody (1HC) - BSA Free

Western Blot: IDO2 Antibody (1HC) [NBP2-21641]

Western Blot: IDO2 Antibody (1HC) [NBP2-21641]

Western Blot: IDO2 Antibody (1HC) [NBP2-21641] - Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK.Lane 3: mouse IDO-1 transgene expressed in 293HEK. IDO2 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band: IDO2 splice variants and isoforms.

Applications for IDO2 Antibody (1HC) - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:50000

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:1500
Application Notes
This product has been tested in western blot and is suitable for use in ELISA and IF. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: IDO2

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 (PubMed 17499941)).(supplied by OMIM)

Long Name

Indoleamine 2,3-dioxygenase 2

Alternate Names

Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2

Entrez Gene IDs

209176 (Mouse)

Gene Symbol

IDO2

UniProt

Q8R0V5 (Mouse)

Additional IDO2 Products

Product Documents for IDO2 Antibody (1HC) - BSA Free

Certificate of Analysis

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Product Specific Notices for IDO2 Antibody (1HC) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IDO2 Antibody (1HC) - BSA Free

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Protocols

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FAQs

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