IFI16 Antibody (2E3) - Azide and BSA Free

Western Blot: IFI16 Antibody (2E3) [H00003428-M03]

Western Blot: IFI16 Antibody (2E3) [H00003428-M03] - Western Blot analysis of IFI16 expression in Hela NE ( Cat # L013V3 ).

Western Blot: IFI16 Antibody (2E3) [H00003428-M03]

Western Blot: IFI16 Antibody (2E3) [H00003428-M03] - Analysis of IFI16 expression in Jurkat.

ELISA: IFI16 Antibody (2E3) [H00003428-M03]

ELISA: IFI16 Antibody (2E3) [H00003428-M03] - Detection limit for recombinant GST tagged IFI16 is approximately 0.3ng/ml as a capture antibody.

Western Blot: IFI16 Antibody (2E3) - Azide and BSA Free [H00003428-M03] -

STING pathway analysis. (A) STING mRNA relative expression in uninfected (CTR), HHV-6A, HHV-6B or HHV-7 infected NK92 cells 3d.p.i. ∗p values Student t test; (B) Western Blot analysis for house-keeping actin (upper blot) and STING (lower blot) expression in uninfected (CTR), HHV-6A, HHV-6B or HHV-7 infected NK92 cells 3d.p.i. For stimulation of the cytoplasmic DNA sensing pathways, we used 2′,3′-cGAMP. The molecular weights were determined by protein ladder (14.4-97.4kDa) (BioRad). Actin was evidenced at 44kDa, STING 35kDa. The images were acquired by Geliance 600 (Perkin Elmer, MA, United States). The complete Western Blots are reported in Supplementary Figure S3. ∗p value Student t test. The histogram represents the STING band intensity in relation with the corresponding actin band. (C) Western Blot analysis for house-keeping actin (upper blot) and cGas, IFI16, TBK1, IRF3, pIRF3Ser396, STAT6, pSTAT6Tyr641 (lower blots) expression in uninfected (CTR), HHV-6A, HHV-6B or HHV-7 infected NK92 cells 3 d.p.i. The molecular weights were determined by protein ladder (25-250kDa; 14.4-97.4 kDa). Actin was evidenced at 44kDa, cGas at 68kDa, IFI16 at 100kDa, TBK1 at 80kDa, IRF3 at 55kDa, STAT6 at 120kDa. The histogram represents the STING band intensity in relation with the corresponding actin band. ∗p value Student t test. The images were acquired by Geliance 600 (Perkin Elmer, MA, United States). The complete Western Blots are reported in Supplementary Figure S3. (D) HHV-6A infected NK92 cells were characterized by immunofluorescence for STING [anti-STING PE Ab (Clone T3-680)], STAT6 [anti-STAT6 FITC (Clone D-1)] and gp116 (Clone 6A5) expression. (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification 100×. Uninfected NK92 cells were used as control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32140147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.