IFN-gamma R2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90223

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IFN-gamma R2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASPSAGFPMDFNVTLRLRAELGALHSAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTAFFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWNKSNIFRVGHLSNIS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFN-gamma R2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IFN-gamma R2 Antibody [NBP1-90223]

Immunocytochemistry/ Immunofluorescence: IFN-gamma R2 Antibody [NBP1-90223]

Immunocytochemistry/Immunofluorescence: IFN-gamma R2 Antibody [NBP1-90223] - Staining of human cell line SiHa shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
IFN-gamma R2 Antibody - BSA Free Immunohistochemistry: IFN-gamma R2 Antibody - BSA Free [NBP1-90223]

Immunohistochemistry: IFN-gamma R2 Antibody - BSA Free [NBP1-90223]

Analysis in human stomach and liver tissues using Anti-IFNGR2 antibody. Corresponding IFNGR2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223] - Staining of human stomach shows high expression.
Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223] - Staining of human liver shows low expression as expected.

Applications for IFN-gamma R2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-90223 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFN-gamma R2

IFN gamma Receptor beta encodes the non-ligand-binding beta chain of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.

Long Name

Interferon gamma Receptor 2

Alternate Names

AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1

Entrez Gene IDs

3460 (Human)

Gene Symbol

IFNGR2

UniProt

P38484

Additional IFN-gamma R2 Products

Product Documents for IFN-gamma R2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IFN-gamma R2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IFN-gamma R2 Antibody - BSA Free

Customer Reviews for IFN-gamma R2 Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 12/23/2015
    IFNgR2 expressin in human mesenchymal stem cells
    IFN-gamma R2 Antibody - BSA Free NBP1-90223

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Protocols

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FAQs for IFN-gamma R2 Antibody - BSA Free

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  • Q: Do you have specific protocol for your IFNgR2 antibody (NBP1-90223) for immunocytochemistry (ICC)? More specifically, how do you recommend fixing the cells?

    A: We have not validated this antibody for the ICC application and, therefore, cannot recommend a protocol. The antibody has been validated in IHC and WB. Your standard protocol for ICC can be found at this link. If you do use this antibody for ICC, you can send your results to us whether negative or positive (if they are tightly controlled) and qualify for the Innovator's reward. Our Innovator’s Reward™ program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product.

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