IgA Antibody (JM10-42)
Novus Biologicals | Catalog # NBP2-66901
Recombinant Monoclonal Antibody
![Western Blot: IgA Antibody (JM10-42) [NBP2-66901]](https://resources.rndsystems.com/images/products/IgA-Antibody-JM10-42-Western-Blot-NBP2-66901-img0004.jpg "Western Blot: IgA Antibody (JM10-42) [NBP2-66901]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JM10-42 expressed in HEK293
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Product Specifications
Immunogen
Recombinant full length protein of Human IgA aa 1-353 / 353. (SwissProt: P01876 Human; SwissProt: P01877 Human)
Localization
Cell membrane, Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgA Antibody (JM10-42)
Western Blot: IgA Antibody (JM10-42) [NBP2-66901]
Western Blot: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of IgA on human plasma lysates using anti-IgA antibody at 1/1,000 dilution.![Immunocytochemistry/ Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901]](https://resources.rndsystems.com/images/products/IgA-Antibody-JM10-42-Immunocytochemistry-Immunofluorescence-NBP2-66901-img0001.jpg "Immunocytochemistry/ Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901]")
Immunocytochemistry/ Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901]
Immunocytochemistry/Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901] - Staining IgA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]](https://resources.rndsystems.com/images/products/IgA-Antibody-JM10-42-Immunohistochemistry-Paraffin-NBP2-66901-img0003.jpg "Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]")
Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]
Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]](https://resources.rndsystems.com/images/products/IgA-Antibody-JM10-42-Immunohistochemistry-Paraffin-NBP2-66901-img0002.jpg "Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]")
Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901]
Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of paraffin-embedded human spleen tissue using anti-IgA antibody. Counter stained with hematoxylin.Applications for IgA Antibody (JM10-42)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgA
Long Name
Immunoglobulin A
Alternate Names
Immunoglobulin A
Gene Symbol
IGHA1
Additional IgA Products
Product Documents for IgA Antibody (JM10-42)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IgA Antibody (JM10-42)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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