Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, ELISA, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Biotin

Antibody Source

Polyclonal Goat
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Product Specifications

Immunogen

Pools of purified homogenous IgE isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure

Reactivity Notes

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this conjugate has not been tested in detail

Specificity

The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Clonality

Polyclonal

Host

Goat

Description

The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.

Applications for Goat anti-Rat IgE Fc Secondary Antibody [Biotin]

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500
Application Notes
.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Reconstitution

Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Formulation

Lyophilized from PBS (pH 7.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgE Fc

Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to be located in extracellular region. Predicted to be part of immunoglobulin complex, circulating. Predicted to be active in external side of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]

Long Name

Immunoglobulin E Fc

Alternate Names

constant region of heavy chain of IgE, immunoglobulin epsilon, immunoglobulin heavy constant epsilon

Additional IgE Fc Products

Product Documents for Goat anti-Rat IgE Fc Secondary Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Rat IgE Fc Secondary Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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FAQs

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