IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25520
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Canine (95%), Porcine (92%). Backed by our 100% Guarantee.
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1982 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human IGF-II/IGF2
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%), Sheep (87%), Bovine (87%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Mouse plasma (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ELISA: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
ELISA: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL1982] (NBP3-25520) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
IGF2 antibody [HL2937] (5 μg/mL)Detection: HRP-conjugated IGF2 antibody [HL1982] (1 μg/mL). Please notice that the detection antibody requires HRP to function as the detection antibody when paired with capture antibody.Please contact us for the conjugation service.Applications for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Sandwich ELISA
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IGF-II/IGF2
Long Name
Insulin-like Growth Factor II
Alternate Names
C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974
Gene Symbol
IGF2
Additional IGF-II/IGF2 Products
Product Documents for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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