Goat anti-Rabbit IgG Fc Secondary Antibody
Novus Biologicals | Catalog # NBP2-60661
![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]](https://resources.rndsystems.com/images/products/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Western-Blot-NBP2-60661-img0013.jpg "Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]")
Key Product Details
Species Reactivity
Rabbit
Applications
Immunohistochemistry, Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Goat anti-Rabbit IgG Fc fragment Secondary Antibody was produced by repeated immunization with rabbit IgG f(c) fragment in goat
Specificity
No reaction was observed against Rabbit IgG F(ab')2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Goat anti-Rabbit IgG Fc Secondary Antibody
Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]
Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody.Lane 1: Rabbit F(c).Load: 50 ng per lane.Primary antibody: Rabbit F(c) antibody at 1:1,000 overnight at 4C.Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 kDa, 25 kDa for Rabbit F(c).![Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]](https://resources.rndsystems.com/images/products/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60661-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]")
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]
Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Immunocytochemistry/Immunofluorescence: [DyLight 488] [NBP1-72943]![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]](https://resources.rndsystems.com/images/products/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Western-Blot-NBP2-60661-img0005.jpg "Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]")
Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]
Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image using the Rhodamine form of this antibody.![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]](https://resources.rndsystems.com/images/products/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0008.jpg "Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]")
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Fluorophore-linked immunosorbent assay: Image using the DyLight 405 format of this antibody.![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]](https://resources.rndsystems.com/images/products/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0009.jpg "Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]")
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 488 format of this antibody.![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]](https://resources.rndsystems.com/images/products/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0010.jpg "Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]")
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 680 format of this antibody.![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]](https://resources.rndsystems.com/images/products/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0011.jpg "Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]")
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661]
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 800 format of this antibody.Applications for Goat anti-Rabbit IgG Fc Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:10000-1:25000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for Goat anti-Rabbit IgG Fc Secondary Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Goat anti-Rabbit IgG Fc Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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