Q: Just wondering if NB7242 anti-llama conjugate will cross react with camel species?

A: Our Goat anti-Llama [HRP] (NB7242) has not yet been tested against other species' IgG. This product may cross-react with other related species. Unfortunately, we do not have any specific data regarding this cross-reactivity. I apologize for the lack of information.

Q: We use the antibody at a very high dilution (1:50,000) which is great, and the 1mg/ml size means it would last for a large number of tests. My queries are: 1. Shelf-life of the product - am I correct in saying 6 months from receipt, or up to 1 year if kept at 4C (which it is)? 2. This is a polyclonal goat antibody. So could you tell me if it is a pool of different goats, or would I be reliant upon one single goat (which then increases my risk of the test being obsolete if a replacement good goat is never found again?). I am after some assurance of product continuity, batch testing of potential replacement goat antibody etc.

A: We only guarantee our products for 6 months, though yes, I am sure it will work for up to a year. The antibody is from a single goat. We have a large volume of serum from the goat that should last us greater than 10 years.

Goat anti-Llama IgG (H+L) Secondary Antibody [HRP]

Novus Biologicals | Catalog # NB7242

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Citations (29)

Product Documents

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Llama

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

HRP

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

This Goat anti-Llama IgG (H+L) Secondary Antibody [HRP] was developed against llama IgG-heavy and light chain.

Specificity

By immunoelectrophoresis and ELISA this Goat anti-Llama IgG (H+L) Secondary Antibody [HRP] reacts specifically with llama IgG and with light chains common to other llama immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Goat anti-Llama IgG (H+L) Secondary Antibody [HRP]

Application

Recommended Usage

ELISA

1:10000-1:100000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:5000

Immunohistochemistry

1:200 - 1:5000

Immunohistochemistry-Paraffin

1:200-1:5000

Western Blot

1:5000 - 1:50000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS) containing 0.2%BSA

Preservative

0.05% Pro-Clean 400

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Llama IgG (H+L) Secondary Antibody [HRP]

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Certificate of Analysis

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Product Specific Notices for Goat anti-Llama IgG (H+L) Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Llama IgG (H+L) Secondary Antibody [HRP]

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