Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Novus Biologicals | Catalog # NB7602

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Cy5 (Excitation = 649 nm, Emission = 666 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed
View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG whole molecule

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) [NB7602] - Western Blot of Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine]. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine] at 1:1000 for 60 min at RT.Block: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.Other band(s): none.. Using the Rhodamine format of this antibody.
Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) [NB7602] - 0.62ng
Primary antibody: none
Secondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine] at 1:1000 for 60 min at RT.
Block: Fluorescent blocking buffer for 60 min at RT.. Using the Rhodamine format of this antibody.

Applications for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Application
Recommended Usage

Flow Cytometry

1:500-1:2500

Fluorophore-linked immunosorbent assay

1:10000-1:50000

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000
Application Notes
This product is designed for ICC/IF, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reviewed Applications

Read 3 reviews rated 4.3 using NB7602 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Format

Pre-adsorbed

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Certificate of Analysis

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Product Specific Notices for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Customer Reviews for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Arun Satelli
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human colonic tissue
    Species: Human
    Verified Customer | Posted 11/04/2014
    Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) NB7602
  • Name: Arun Satelli
    Application: Flow Cytometry
    Sample Tested: Human colon cancer cell line HT29
    Species: Human
    Verified Customer | Posted 10/16/2014
    Anti-mouse Cy5 Flow
    Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) NB7602
  • Name: Arun Satelli
    Application: Immunocytochemistry
    Sample Tested: Human colon cancer cell line HT29
    Species: Human
    Verified Customer | Posted 10/16/2014
    Anti-mouse Cy5 ICC
    Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) NB7602

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