Rabbit anti-Sheep IgG (H+L) Secondary Antibody

Name: Rabbit anti-Sheep IgG (H+L) Secondary Antibody
Brand: Novus Biologicals
SKU: NBP1-72704
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-72704

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Sheep IgG whole molecule

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Sheep IgG (H+L) Secondary Antibody

Immunocytochemistry/ Immunofluorescence: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704]

Immunocytochemistry/Immunofluorescence: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - Immunocytochemistry/Immunofluorescence: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72996] Image from the DyLight 488 version of this antibody.

Dot Blot: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - Sheep IgG. Load: 200 ug, 66.7 ug, 22.2 ug, 7.41 ug or 2.47 ug as indicated. Primary antibody: N/A. Secondary antibody: Alkaline Phosphatase Conjugated Rabbit anti-Sheep IgG Antibody. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 1 hour.

Fluorophore-linked immunosorbent assay: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704]

Fluorophore-linked immunosorbent assay: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - Using the DyLight 405 format of this antibody.

ELISA: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704]

ELISA: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - ELISA results of purified Rabbit anti-Sheep IgG (H+L) Secondary Antibody [Unconjugated] tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 ug of Sheep IgG (purple line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed),, and TMB substrate.

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Applications for Rabbit anti-Sheep IgG (H+L) Secondary Antibody

Application

Recommended Usage

ELISA

1:50000 - 1:200000

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:2000 - 1:10000

Application Notes

This product has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Rabbit anti-Sheep IgG (H+L) Secondary Antibody

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Product Specific Notices for Rabbit anti-Sheep IgG (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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