Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Dot Blot

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Rabbit IgG
View all IgG2a Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG2a heavy chain

Specificity

Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum

Scientific Data Images

Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72673] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [Alkaline Phosphatase].Lane 1: Mouse IgG2a.Lane 2: none. Load: 50ng per lane.Primary antibody: none.Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [Alkaline Phosphatase] at 1:1,000 at 4C.Block for 30 min at RT.Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT.Predicted/Observed size: 55kDa for Mouse IgG2a.
Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72673]
Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase]

Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase]

Dot Blot of Mouse IgG2a Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng Primary antibody: none Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 for 60 min at RT. Block for 60 min at RT Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.

Applications

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2500
Application Notes
This product has been tested by ELISA, dot blot, and western blot This antibody is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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