Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # IGG2a/6723

Format

Azide and BSA Free
View all IgG2A Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human IgG2a protein

Localization

None

Specificity

To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Non-species specific IgG2a Isotype Control (IGG2a/6723)

Non-species specific IgG2a Isotype Control (IGG2a/6723)

Immunohistochemistry-Paraffin: Non-species specific IgG2a Isotype Control (IGG2a/6723) [NBP3-27042] -

Formalin-fixed, paraffin-embedded human tonsil stained with Non-species specific IgG2a Isotype Control (IGG2a/6723). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for Non-species specific IgG2a Isotype Control (IGG2a/6723)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml for 30 minutes at RT

Western Blot

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Non-species specific IgG2a Isotype Control (IGG2a/6723)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Non-species specific IgG2a Isotype Control (IGG2a/6723)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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