Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG3 Clone # MG3
View all IgG3 Isotype Controls »

Product Specifications

Specificity

Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product has been prepared from serum-free tissue culture supernate by protein A chromatography

Scientific Data Images for Mouse IgG3 Isotype Control (MG3) [FITC]

Dot Blot: Mouse IgG3 Isotype Control (MG3) [FITC] [NBP1-96847] - Mouse IgG3 Protein [Fluorescein] [NBP1-96847] - A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ug was spotted onto 0.45 um nitrocellulose.
Mouse IgG3 Isotype Control (MG3) [FITC]

Mouse IgG3 Isotype Control (MG3) [FITC]

Dot Blot showing the detection Fluorescein conjugated Mouse IgG3 subclass. A three-fold serial dilution of Mouse IgG3 Isotype Control (MG3) [FITC]starting at 200ng was spotted onto 0.45 ?m nitrocellulose and imaged using the Bio-Rad VersaDoc(R) 4000 MP.

Applications for Mouse IgG3 Isotype Control (MG3) [FITC]

Application
Recommended Usage

Dot Blot

1:100 - 1:2000

ELISA

1:100 - 1:2000

Flow Cytometry

1:1000 - 1:5000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G3

Alternate Names

DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809

Gene Symbol

IGHG3

Additional IgG3 Products

Product Documents for Mouse IgG3 Isotype Control (MG3) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mouse IgG3 Isotype Control (MG3) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Customer Reviews for Mouse IgG3 Isotype Control (MG3) [FITC]

There are currently no reviews for this product. Be the first to review Mouse IgG3 Isotype Control (MG3) [FITC] and earn rewards!

Have you used Mouse IgG3 Isotype Control (MG3) [FITC]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Isotype Controls