Mouse IgG3 Lambda Isotype Control (MG3L)
Novus Biologicals | Catalog # NBP1-96977
![SDS-PAGE: Mouse IgG3 Lambda Isotype Control (MG3L) [NBP1-96977]](https://resources.rndsystems.com/images/products/Mouse-IgG3-Lambda-Isotype-Control-MG3L-SDS-Page-NBP1-96977-img0001.jpg "SDS-PAGE: Mouse IgG3 Lambda Isotype Control (MG3L) [NBP1-96977]")
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Description
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Mouse IgG3 Lambda Isotype Control (MG3L)
SDS-PAGE: Mouse IgG3 Lambda Isotype Control (MG3L) [NBP1-96977]
SDS-Page: Mouse IgG3 Lambda Isotype Control (MG3L) [NBP1-96977] - SDS PAGE Results of Mouse IgG3 Lambda isotype control (MG3L). Lane 1: Mouse IgG3 Lambda isotype control (MG3L) Reduced [5ug]. Lane 2: Unstained Molecular weight marker. Lane 3: Mouse IgG3 Lambda isotype control (MG3L) Non-Reduced [5ug].4-20% Gel.Applications for Mouse IgG3 Lambda Isotype Control (MG3L)
Flow Cytometry
Fluorophore-linked immunosorbent assay
Immunocytochemistry/ Immunofluorescence
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
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Product Documents for Mouse IgG3 Lambda Isotype Control (MG3L)
Certificate of Analysis
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Product Specific Notices for Mouse IgG3 Lambda Isotype Control (MG3L)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
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- Troubleshooting Guide: ELISA
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse IgG3 Lambda Isotype Control (MG3L)
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Q: Please provide information about Mouse IgG3 Isotype Control (MG3L)(#NBP1-96977). Has the light chain of this Ab been identified? If so, kappa or lambda?
A:
About the subclass of this isotype control, the following statements were derived from the databases of our manufacturing lab for the product: Application Note: Mouse IgG3 lambda isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. Purity/Specifity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgM and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition confirmed by RID. That being said, NBP1-96977 has the lambda light chain, and only reacted with IgG3 itself at the QC tests. Unlike IgG1 and IgG2, which have many subclasses, IgG3 does not (https://absoluteantibody.com/antibody-resources/antibody-overview/antibody-isotypes-subtypes/, https://www.jacksonimmuno.com/technical/products/groups/subclass-specific, https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.0300-9475.2004.01362.x, and others). In summary, NBP1-96977 is a mouse IgG3 Isotype Control (against IgG3 only no subclass) with the lambda light chain.