Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IgM Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Human IgM whole molecule

Specificity

No reaction was observed against other human heavy or light chain proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Human IgM Heavy Chain Secondary Antibody

Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Secondary Antibody [DyLight 488] [NBP1-72860]
Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 405 format of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 488 format of this antibody.
ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 680 format of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 800 format of this antibody.
ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670]

ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - ELISA results of purified Goat anti-Human IgM Heavy Chain Secondary antibody tested against purified human IgM [RED line], human IgG F(ab')2 {PURPLE line], human IgG Fab [BLUE line], human IgG F(c) [GREEN line]. Each well was coated in duplicate with 1.0 ug of IgM, IgG F(ab')2, IgG Fab, IgG F(c). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Donkey-anti-Goat IgG HRP conjugate 1:12, 000, and TMB substrate.

Applications for Goat anti-Human IgM Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:10000
Application Notes
This product has been tested by ELISA and SDS-PAGE and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Goat anti-Human IgM Heavy Chain Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Goat anti-Human IgM Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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