Ikaros/IKZF1 Antibody (4S10I3)

Novus Biologicals | Catalog # NBP3-16223

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4S10I3 expressed in HEK293
View all Ikaros Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Ikaros/IKZF1 (Q13422). AEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ikaros/IKZF1 Antibody (4S10I3)

Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]

Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]

Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Analysis of extracts of various cell lines, using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Ikaros/IKZF1 Antibody (4S10I3)

Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] -

Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Immunofluorescence analysis of Jurkat cells using Ikaros/IKZF1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Ikaros/IKZF1 Antibody (4S10I3)

Chromatin Immunoprecipitation: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] -

Chromatin Immunoprecipitation: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros/IKZF1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for Ikaros/IKZF1 Antibody (4S10I3)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5μg antibody for 10μg-15μg of Chromatin

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ikaros

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs)

Long Name

DNA-binding protein Ikaros

Alternate Names

IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1

Gene Symbol

IKZF1

Additional Ikaros Products

Product Documents for Ikaros/IKZF1 Antibody (4S10I3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ikaros/IKZF1 Antibody (4S10I3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Ikaros/IKZF1 Antibody (4S10I3)

There are currently no reviews for this product. Be the first to review Ikaros/IKZF1 Antibody (4S10I3) and earn rewards!

Have you used Ikaros/IKZF1 Antibody (4S10I3)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies