IkB-alpha Antibody - BSA Free

Name: IkB-alpha Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB120-22071
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NB120-22071

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 34-48 of human IkB-alpha was used as the immunogen.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28765552). Cross-reacts with Human. It will cross-react with mouse and rat IkB alpha, based on BLAST.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IkB-alpha Antibody - BSA Free

Western Blot: IkB-alpha Antibody [NB120-22071]

Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.

Immunohistochemistry-Paraffin: IkB-alpha Antibody [NB120-22071]

Immunohistochemistry-Paraffin: IkB-alpha Antibody [NB120-22071] - Analysis of human breast using IkB-alpha antibody at 10 ug/ml.

Applications for IkB-alpha Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1 - 3 ug/ml

Application Notes

In Jurkat, a 40 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkB alpha is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkB alpha.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Entrez Gene IDs

25493 (Rat)

Gene Symbol

NFKBIA

UniProt

P25963

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Product Specific Notices for IkB-alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.