IKBKAP Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35099

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1093-1332 of human IKBKAP (NP_003631.2).

Sequence:
LRLVYKYNRLDIIETNVKPSILEAQKNYMAFLDSQTATFSRHKKRLLVVRELKEQAQQAGLDDEVPHGQESDLFSETSSVVSGSEMSGKYSHSNSRISARSSKNRRKAERKKHSLKEGSPLEDLALLEALSEVVQNTENLKDEVYHILKVLFLFEFDEQGRELQKAFEDTLQLMERSLPEIWTLTYQQNSATPVLGPNSTANSIMASYQQQKTSVPVLDAELFIPPKINRRTQWKLSLLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IKBKAP Antibody - BSA Free

IKBKAP Antibody

Immunohistochemistry: IKBKAP Antibody [NBP3-35099] -

Immunohistochemistry: IKBKAP Antibody [NBP3-35099] - Immunohistochemistry analysis of paraffin-embedded Rat stomach using IKBKAP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
IKBKAP Antibody

Immunohistochemistry: IKBKAP Antibody [NBP3-35099] -

Immunohistochemistry: IKBKAP Antibody [NBP3-35099] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using IKBKAP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
IKBKAP Antibody

Immunohistochemistry: IKBKAP Antibody [NBP3-35099] -

Immunohistochemistry: IKBKAP Antibody [NBP3-35099] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using IKBKAP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
IKBKAP Antibody

Western Blot: IKBKAP Antibody [NBP3-35099] -

Western Blot: IKBKAP Antibody [NBP3-35099] - Western blot analysis of various lysates using IKBKAP Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for IKBKAP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKBKAP

The protein encoded by the IKBKAP gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. (provided by RefSeq)

Alternate Names

DYS, elongator complex protein 1, ELP1DKFZp781H1425, FD, FLJ12497, IKAPdysautonomia (Riley-Day syndrome, hereditary sensory autonomic neuropathy typeIII), IkappaB kinase complex-associated protein, IKI3, IKK complex-associated protein, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinasecomplex-associated protein, p150, TOT1

Gene Symbol

ELP1

Additional IKBKAP Products

Product Documents for IKBKAP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IKBKAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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