IKK epsilon/IKBKE [p Thr501] Antibody [DyLight 350]

Name: IKK epsilon/IKBKE [p Thr501] Antibody [DyLight 350]
Brand: Novus Biologicals
SKU: NBP1-77829UV
Price: 619 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-77829UV

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Product Documents

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

IKK epsilon/IKBKE phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH. (Uniprot: Q14164)

Reactivity Notes

This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKK epsilon/IKBKE. Reactivity with non-phosphorylated IKK epsilon/IKBKE is minimal. Cross reactivity with pT501 phosphorylated IKK epsilon/IKBKE from mouse, rat or other species has not been determined.

Modification

p Thr501

Specificity

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for IKK epsilon/IKBKE [p Thr501] Antibody [DyLight 350]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IKK epsilon

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.

Long Name

IkB Kinase epsilon

Alternate Names

IKBKE, IKK-I

Gene Symbol

IKBKE

Additional IKK epsilon Products

Product Documents for IKK epsilon/IKBKE [p Thr501] Antibody [DyLight 350]

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Certificate of Analysis

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Product Specific Notices for IKK epsilon/IKBKE [p Thr501] Antibody [DyLight 350]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.