IKK gamma Antibody (72C627) - BSA Free

Name: IKK gamma Antibody (72C627) - BSA Free
Brand: Novus Biologicals
SKU: NB100-56542
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-56542

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Citations (8)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 72C627

Format

BSA Free

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Product Specifications

Immunogen

This antibody was raised against a His-tagged full-length human IKK3/IKKg protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for IKK gamma Antibody (72C627) - BSA Free

Western Blot: IKK gamma Antibody (72C627) [NB100-56542]

Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.

Simple Western: IKK gamma Antibody (72C627) [NB100-56542]

Simple Western: IKK gamma Antibody (72C627) [NB100-56542] - Simple Western lane view shows a specific band for IKK gamma in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for IKK gamma Antibody (72C627) - BSA Free

Application

Recommended Usage

Simple Western

1:200

Western Blot

2-5 ug/ml

Application Notes

A 48 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK gamma

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg. This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb.

Long Name

IkB Kinase gamma

Alternate Names

FIP3P, IKBKG, IKKAP1, NEMO

Entrez Gene IDs

8517 (Human); 16151 (Mouse)

Gene Symbol

IKBKG

UniProt

Q9Y6K9

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Product Specific Notices for IKK gamma Antibody (72C627) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.