Q: Does viral IL-10 cross-react in bioassay?

A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

IL-10 Antibody (3C12C12) - BSA Free

Novus Biologicals | Catalog # NB100-2185

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 3C12C12

Format

BSA Free

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Product Specifications

Immunogen

Purified recombinant fragment of human IL-10 expressed in E. Coli.

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for IL-10 Antibody (3C12C12) - BSA Free

Western Blot: IL-10 Antibody (3C12C12)BSA Free [NB100-2185]

Western Blot: IL-10 Antibody (3C12C12) [NB100-2185] - Analysis using IL10 mouse mAb against IL10 recombinant protein.

Applications for IL-10 Antibody (3C12C12) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Western Blot

1:500-1:2000

Application Notes

Anti- IL-10 recognizes recombinant human IL-10 by Western Blot and ELISA.

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascitic fluid

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-10

Interleukine 10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down- regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human)

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for IL-10 Antibody (3C12C12) - BSA Free

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Certificate of Analysis

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Product Specific Notices for IL-10 Antibody (3C12C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.